Figure 6
Confirmation of mRNA gene expression changes in the regression experiment using the porcine model of dietary NAFLD development. Analyses by RT-qPCR of previously observed differentially expressed genes (A). Data (mean ± SD) represent arbitrary units normalized to UBA52 expression. Statistical analyses were carried out by Mann–Whitney U test. *p < 0.05, **p < 0.01 and ***p < 0.005. Venn diagram analysis showing the transcripts displaying increased expression in the steatotic condition (yellow area) of progression and regression experiments (B). Significant (P < 0.001) association between hepatic lipid droplet content and LGALS3 expression (C), hepatic triglycerides and gene expressions (D) and hepatic inflammation (CD68 immunostaining) and gene expressions (E). Red colour denotes a positive association while blue a negative one. Correlations were calculated according to the Spearman’s rho test.
