Table 1 Selected hepatic transcripts differentially upregulated in male pigs fed the steatotic diet according to RNAseq at the level of FDR < 0.001 and FPKM ≥ 0.3.
Gene ID | Symbol | Initial state (FPKM) | Steatosis (FPKM) | Log2 FC | ||
|---|---|---|---|---|---|---|
Up-regulated | ||||||
Apoptosis, cell adhesion | 100,038,033 | Galectin-3 | LGALS3 | 4.3 ± 1.8 | 274.9 ± 81.9 | 6.0 |
Growth delay and reduction of metastatic potential | 100,049,669 | Glycoprotein NMB | GPNMB | 24 ± 6 | 776 ± 303 | 5.0 |
Activation of macrophages | 100,520,753 | Macrosialin | CD68 | 1 ± 0.5 | 16 ± 9 | 4.9 |
Transport of bile acids | 100,525,144 | Solute carrier family 51 subunit beta | SLC51B | 0.3 ± 0.1 | 8.0 ± 4.8 | 4.8 |
Production of interferon-gamma and interleukin-12 | 397,087 | Osteopontin | SPP1 | 6.1 ± 2.5 | 151 ± 73 | 4.6 |
Down-regulated | ||||||
Cu and Zn transport | 397,123 | Metallothionein-3 | MT3 | 8.6 ± 1.6 | 0.4 ± 0.1 | − 4.3 |
Protein folding | 100,514,482 | Anterior gradient protein 2 | AGR2 | 8 ± 13 | 0.6 ± 0.1 | − 3.7 |
Resistance to TNF-induced apoptosis | 100,302,365 | Phosphatidylethanolamine-binding protein 4 | PEBP4 | 2.6 ± 0.8 | 0.3 ± 0.3 | − 3.0 |
Nucleotide import | 100,156,347 | Calcium-binding mitochondrial carrier protein | SLC25A25 | 64 ± 71 | 8.3 ± 1.3 | − 2.9 |
Breakdown of phosphoethanolamine | 100,519,324 | Ethanolamine-phosphate phospho-lyase | ETNPPL | 23 ± 16 | 3.2 ± 1.8 | − 2.9 |
Copper transport | 100,037,920 | Metallothionein-1E | MT1D | 9491 ± 1350 | 1318 ± 775 | − 2.8 |
Oxidation of squalene | 100,113,409 | Squalene epoxidase | SQLE | 34.6 ± 7 | 5.1 ± 8.2 | − 2.8 |
Binding of divalent heavy metal ions | 102,166,944 | Metallothionein-1A | MT1A | 4311 ± 913 | 644 ± 325 | − 2.7 |
Copper ion binding | 100,151,998 | Monooxygenase, DBH-like 1 | MOXD1 | 1.8 ± 0.5 | 0.3 ± 0.1 | − 2.7 |
Post-transcriptional repressor | 100,157,783 | Nanos C2HC-type zinc finger 1 | NANOS1 | 1.3 ± 1.1 | 0.2 ± 0.0 | − 2.6 |
