Figure 2
From: CCN2-induced lymphangiogenesis is mediated by the integrin αvβ5–ERK pathway and regulated by DUSP6
Expression levels of lymphatic endothelial markers in CCN2-treated LECs. Mouse primary cultured LECs were treated with 0, 12.5, 25, 50, 100 or 200 ng/mL CCN2 for 3 h. (A) mRNA expression levels of Lyve1, Podoplanin and Prox1 were analyzed by quantitative RT-PCR. (B) Vegfc, Vegfd, Kdr (encoding VEGFR2) and Flt4 (encoding VEGFR3) mRNA levels were analyzed by quantitative RT-PCR. Actb was used as an internal control, and gene expression levels were expressed relative to Actb mRNA. One-way ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001 versus 0 ng/mL CCN2. †††p < 0.01 versus 100 ng/mL CCN2. (C) LECs were treated with 100 ng/ml CCN2 for 24 h, and protein expression levels of LYVE-1, VEGFR3, and Podoplanin were detected by Western blotting. One-way ANOVA, **p < 0.01 versus 0 h. Student t test, ns; not significant.
