Figure 3
From: Label-free imaging flow cytometry for analysis and sorting of enzymatically dissociated tissues
Detection and separation of cell aggregates. (A) Examples of images captured during sorting. A single contour is detected in the upper image, while three contours are detected in the lower image. Sorting trigger is omitted when more than one contour is detected. Scale bar: 20 µm. (B) The histogram shows the probability to have n cells in a unit volume. The chance of having more than one cell in the sorting region during a sorting pulse is 26.4% (blue) and 6.2% (red) for an initial cell concentration of 50 million cells/ml and 20 million cells/ml, respectively. (C) Plot shows the measurement time and number of captured events of a measurement of Nrl-eGFP mice retina cells. Color code indicates the time difference between two events. While most of the time, events are captured with a time difference of > 0.02 s, during an avalanche, each captured frame contains cells, resulting in a time difference of approximately 0.00033 s = 0.33 ms. Scale bar: 10 µm.
