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Figure 1

From: Construction and validation of safe Clostridium botulinum Group II surrogate strain producing inactive botulinum neurotoxin type E toxoid

Figure 1

General characterization of Clostridium botulinum Beluga Ei strain in comparison to C. botulinum Beluga wild-type (Beluga WT) cultivated in cooked meat medium-tryptone-peptone-glucose-yeast extract (CMM-TPGY) broth during 96 h. (a) Optical density of the cultures measured at 600 nm wavelength (OD600). (b) Morphology of bacterial cells evaluated with phase-contrast microscopy during the late-logarithmic growth phase (9 h) and the late stationary phase (72 h). Concentrations of Beluga WT and Beluga Ei viable cells (c) and heat-resistant spores (d) in the culture measured during the bacterial growth. All data were obtained from three parallel biological replicates. *, cell concentration of a mutant was significantly (p < 0.05) higher than that of WT. Error bars indicate the highest and the lowest values of three biological replicates measured.

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