Figure 2

BoNT/E and BoNT/Ei concentrations and distribution in Clostridium botulinum Beluga wild-type (Beluga WT) (a) and Beluga Ei (b) cultures determined with BoNT/E-directed sandwich enzyme-linked immunosorbent assay (ELISA). Error bars indicate the highest and the lowest concentrations measured of the three parallel biological replicates.