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Figure 3

From: Construction and validation of safe Clostridium botulinum Group II surrogate strain producing inactive botulinum neurotoxin type E toxoid

Figure 3

Restriction digestion-based verification of the presence of designed nucleotide modifications within bont locus of Clostridium botulinum Beluga wild-type (Beluga WT), Beluga Ei 1st generation (Beluga Ei G1) and Beluga Ei 25th generation (Beluga Ei G25). (a) Schematic representation of the restriction digestion-based Beluga Ei strain validation. Altered nucleotides introducing the restriction sites are underlined. (b) Agarose gel electrophoresis visualizing the PCR amplicons after restriction digestion. Full-length agarose gel is presented in Supplementary Figure S3.

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