Figure 4 | Scientific Reports

Figure 4

From: Construction and validation of safe Clostridium botulinum Group II surrogate strain producing inactive botulinum neurotoxin type E toxoid

Figure 4

Botulinum neurotoxin (BoNT) type E-directed Western blot (upper picture) and the representative Coomassie-stained polyacrylamide gel (lower picture) validating the correct trypsin-cleavage of BoNT/E and BoNT/Ei produced by 1st and 25th generations of the Clostridium botulinum Beluga Ei strain (Beluga Ei G1 and Beluga Ei G25). Samples were diluted in standard phosphate-gelatin buffer prior to addition of trypsin. In the absence of trypsin gelatin creates strong background visible in Coomassie-stained gel. Full-length images of the Western blot membrane and of the Coomassie-stained gel are presented in Supplementary Figures S4 and S5, respectively.

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