Figure 5
Expression of hemoglobin proteins in (A) buffy coat, (B) plasma, and (C) serum (HBA1 = Hemoglobin Subunit Alpha, HBB = Hemoglobin Subunit Beta, HBD = Hemoglobin Subunit Delta) as a relative measure of hemolysis. (A) HBA1 protein expression comparison between hemolysis score of 0 versus 1 in buffy coat with adjusted p value of 0.0014, comparison between 0 versus 2 with adjusted p value of 1.29e−38 and comparison between 0 versus 3 with adjusted p value of 2.29e−47. HBD protein comparison between 0 versus 1 with adjusted p value of 1.40E−05, comparison of 0 versus 2 with adjusted p value of 6.23e−44 and comparison of 0 versus 3 with adjusted p value of 7.28E−49. HBB protein comparison between 0 versus 1 with adjusted p value of 0.0005, comparison of 0 versus 2 with adjusted p value of 1.13e−40 and comparison of 0 versus 3 with an adjusted p value of 2.62e−47. Samples were grouped into hemolysis score 1 and 2+ for plasma and serum due to low number of score 3 and 4 samples in these matrices. (B) For plasma shows HBA1 protein comparison of hemolysis score 1 versus 2 with adjusted p value of 6.65E−09, HBB protein comparison 1 versus 2 with adjusted p value of 4.24e−10, and HBD protein comparison of 1 versus 2 with adjusted p value of 0.00027. C For serum shows HBA1 protein comparison 1 versus 2 with adjusted p value of 4.16e−22, HBB protein comparison 1 versus 2 with adjusted p value of 1.15e−23, and HBD protein comparison of 1 versus 2 with adjusted p value of 3.56e−09.
