Figure 2

Results of the flow cytometry and RT-qPCR analysis of VPA-treated hESCs. (a) The percentage of eGFP negative cells depicted in function of an increasing concentration (in mM) of VPA as determined with flow cytometry. (b) The Calibrated Normalized Relative Quantity (CNRQ) of NES and POU5F1, an early (neuro)ectoderm differentiation and a pluripotency marker respectively, represented in function of increasing VPA-concentrations (mM).