Figure 3

High glucose concentrations decrease Nrf2 and Keap1 levels in MC. Western blot analysis of Nrf2 and Keap1 expression in homogenates from MC exposed to NG (Ctr) or HG for different time periods (1–48 h). The upper part, representative western blot (a). The lower part, densitometric quantification of Nrf2 (b) and Keap1 (c) levels. The relative expression levels were normalized using actin. Values are the mean ± SEM (n = 7 per group) carried out in duplicate. NG, normal glucose; HG, high glucose. *p < 0.05 with respect to NG, ***p < 0.001 with respect to NG. Full-size blots are presented in Supplementary Fig. 3.