Figure 5 | Scientific Reports

Figure 5

From: High glucose concentrations induce oxidative stress by inhibiting Nrf2 expression in rat Müller retinal cells in vitro

Figure 5

High glucose concentrations alter the expression of antioxidant enzymes and GSH levels. MC were exposed to HG for the indicated time intervals. mRNA levels of GCLc (a), SOD2 (b), TXN (c) and Nrf2 (f). The relative mRNA levels were normalized using ACT (actin). Values are the mean ± SEM (n = 4 per group) carried out in duplicate. (d) Glutathione (GSH) levels are expressed as nmol/mg protein. (e) Catalase activity is expressed as U/mg protein, as described in Methods. Values are the mean ± SEM (n = 5 per group) carried out in duplicate. NG, normal glucose; HG, high glucose. *p < 0.05 with respect to NG, **p < 0.01 with respect to NG, ***p < 0.001 with respect to NG.

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