Figure 3
From: Cold shock induces a terminal investment reproductive response in C. elegans
TAX-2; TAX-4-mediated thermosensation is required for lipid relocalization following cold shock. (a) tax-2(p671); tax-4(p678) double loss-of-function mutants were cold shocked and recovered while monitoring survival rates (n ≥ 120 worms per condition). (b) At 12 h post-CS or nonCS control, tax-2(p671); tax-4(p678) were Nile Red lipid-stained and the average fluorescence per worm was quantified (Kruskal–Wallis test: H = 83.83, P < 0.0001; Dunn’s Multiple Comparison test: ****P < 0.0001). (c) Representative images of Nile Red staining show retention of fluorescence in the intestines of tax-2; tax-4 mutants. (d) Number of embryos (Kruskal–Wallis test: H = 171.1, P < 0.0001; Dunn’s Multiple Comparison test: *P = 0.0484, ****P < 0.0001) and (e) percent fluorescent internal embryos (Kruskal–Wallis test: H = 100.1, P < 0.0001; Dunn’s Multiple Comparison test: ****P < 0.0001) were quantified per worm from Nile Red images (n ≥ 24 worms per condition for b-e; error bars are mean ± s.e.m.).
