Figure 5
From: Cold shock induces a terminal investment reproductive response in C. elegans
VIT-2-regulated vitellogenesis during recovery promotes lipid relocalization and impairs survival. (a) vit-2(ok3211) loss-of-function mutants were cold shocked and recovered while monitoring survival rates (n ≥ 130 worms per condition). (b) At 12 h post-CS or nonCS, vit-2(ok3211) were Nile Red lipid-stained and average fluorescence per worm quantified (n ≥ 31 worms per condition; Kruskal–Wallis test: H = 75.18, P < 0.0001; Dunn’s Multiple Comparison test: ****P < 0.0001). Error bars are mean ± s.e.m. (c) Images of Nile Red Staining show intestinal lipid retention in vit-2 mutants. (d) Number of embryos (Kruskal–Wallis test: H = 169.9, P < 0.0001; Dunn’s Multiple Comparison test: *P = 0.0149, ****P < 0.0001) and (e) percent of fluorescent internal embryos (Kruskal–Wallis test: H = 101.5, P < 0.0001; Dunn’s Multiple Comparison test: ****P < 0.0001) were quantified per worm from Nile Red images (n ≥ 31 for b–e; error bars are mean ± s.e.m.).
