Figure 7
From: Cold shock induces a terminal investment reproductive response in C. elegans
Cold shock response is a form of terminal investment. (a) glp-1(e2141) and glp-1(q231) were grown at the restrictive temperature (25 °C) to induce sterility and then habituated at 20 °C for 4 h. Worms were then cold shocked or mock shocked (nonCS) and recovered at 20 °C for 24 h and phenotypes were scored (n ≥ 197 worms for all conditions; Chi-Squared Test for Homogeneity, P < 0.0001 for N2 CS vs. e2141 CS or q231 CS). (b) Young adult hermaphrodite N2 and vit-2(ok3211) (P0) were cold shocked or mock shocked (nonCS) for 4 h and recovered for 16 h. Embryos from P0 treatments were collected from 16 to 18 h and cold shocked for 24 h. Embryos were allowed to recover 24 h at 20 °C and then the number of hatched embryos was quantified. Data points represent rates as percent hatched for a plate containing 50–100 embryos (n ≥ 10 plates per condition; 2-way ANOVA (F(3,32) = 20.87, P < 0.0001) with Tukey multiple comparison test (****P < 0.0001). Bars are mean ± s.e.m. (c) Performed as in b with N2 and vit-5 (ok3239) worms (n = 10 plates per condition; 2-way ANOVA (F(3,27) = 35.28, P < 0.0001) and Tukey multiple comparison test (****P < 0.0001).
