Figure 3
From: VDR regulates simulated microgravity-induced atrophy in C2C12 myotubes
VDR deficiency does not affect myotube differentiation. (a) Diagram showing Vdr deletion using the CRISPR-Cas9 system. (b) Western blot of VDR and β-Actin in C2C12 myotubes confirming that VDR expression was absent in Vdr KO cells. (c) Phase contrast images of control and Vdr KO C2C12 cells after differentiation for 3 or 5 days. Scale bars; 500 μm. (d) Distribution of myotube lengths. Data are means ± s.d. Mean numbers of myotubes within the indicated length ranges per 100 myotubes in control and Vdr KO C2C12 cells after differentiation for 3 or 5 days. Data are means ± s.d. Two-way ANOVA and multiple Mann–Whitney tests for multiple comparisons. ns: no significant. (e) Myod1, Myog, Myh3 and Myh4 mRNA expression in control and Vdr KO C2C12 cells after differentiation for 0, 3, or 5 days. Data are means ± s.d. Two-way ANOVA and multiple Mann–Whitney tests for multiple comparisons. ns: not significant.
