Table 1 Metadata and DNA quantity and quality of samples used in this study.
Sample description | Sample ID | Sequencing date | Extraction methods | Kit | Flowcell ID | Number of barcodes used per flowcell | DNA quantity and quality | ||
|---|---|---|---|---|---|---|---|---|---|
DNA concentration (ng/μL) | A260/A280 | A260/A230 | |||||||
Moderately diseased plant | S1 | Nov22 | Sonication | DNeasy® PowerWater® | FAK95928 | 4 | 317.0 | 1.90 | 1.98 |
S2 | Nov22 | Sonication | DNeasy® PowerSoil® Pro | 479.5 | 1.93 | 1.74 | |||
S3 | Nov22 | Sonication | ZymoBIOMICSâ„¢ DNA Miniprep | 403.5 | 1.92 | 1.96 | |||
G1 | Nov22 | Grinding | ZymoBIOMICSâ„¢ DNA Miniprep | 76.0 | 1.89 | 1.72 | |||
G2 | Nov24 | Grinding | DNeasy® PowerPlant® Pro | FAK96453 | 5 | 163.1 | 1.66 | 0.74 | |
G3 | Nov24 | Grinding | Invisorb® Spin Plant Mini | 103.0 | 1.71 | 0.57 | |||
G4 | Nov24 | Grinding | OmniPrepâ„¢ | 203.1 | 1.73 | 0.69 | |||
G5 | Nov24 | Grinding | OmniPrepâ„¢ with RNAse | 277.8 | 1.81 | 1.95 | |||
G6 | Nov24 | Grinding | Gentra® Puregene® | 314.6 | 1.45 | 0.45 | |||
Severely diseased plant (Batch 1) | S4 | Dec12 | Sonication | DNeasy® PowerWater® | FAN08223 | 4 | 151.0 | 1.89 | 2.02 |
S5 | Dec12 | Sonication | DNeasy® PowerSoil® Pro | 98.0 | 1.90 | 0.83 | |||
G7 | Dec12 | Grinding | DNeasy® PowerPlant® Pro | 76.8 | 0.89 | 0.20 | |||
G8 | Dec12 | Grinding | Invisorb® Spin Plant Mini | 135.1 | 1.77 | 1.49 | |||
G9 | Dec17 | Grinding | OmniPrepâ„¢ | FAN08200 | 5 | 170.3 | 2.10 | 2.23 | |
Severely diseased plant (Batch 2) | G10 | Dec17 | Grinding | OmniPrepâ„¢ | 1132.5 | 2.10 | 1.88 | ||
G11 | Dec17 | Grinding | OmniPrepâ„¢ diluted 10 times and treated with Rnase | 110.1 | 1.96 | 0.49 | |||
G12 | Dec17 | Grinding | ZymoBIOMICSâ„¢ DNA Miniprep | 77.4 | 2.19 | 0.08 | |||
G13 | Dec17 | Grinding | Gentra® Puregene® | 349.0 | 1.57 | 0.55 | |||
Healthy plant | NC | Negative control | Grinding | ZymoBIOMICSâ„¢ DNA Miniprep | FAO99127 | 2 | 82.9 | 1.87 | 1.53 |
