Figure 2
From: Intracellular glucose starvation affects gingival homeostasis and autophagy
Low glucose concentrations affect the capability of wound-healing on human gingival fibroblast (HGnFs). (a) The process of wound healing assay was photographed at 0 and 24 h. (b) The data of wound healing assay is presented as the ratio of the final to the initial scratch size. (c) HGnFs cell migration under low glucose was indicated by fluorescence intensity. FBS (10%) was used as the positive control. (d and e) Type I collagen synthesis by HGnFs under low glucose concentrations was measured after incubation for 72 and 120 h. HGnFs were photographed after being incubated with fluorescently labeled secondary antibody and stained with DAPI. The data of the synthesis of type I collagen are presented as the ratio of the stained area. (Scale bars: 500 μm; A significant increase compared with the control was described as *P < 0.05, ** P < 0.01; A significant decrease compared with the control was described as ††P < 0.01).
