Figure 6 | Scientific Reports

Figure 6

From: Metformin sensitizes leukemic cells to cytotoxic lymphocytes by increasing expression of intercellular adhesion molecule-1 (ICAM-1)

Figure 6

Metformin mainly favors leukemic cell killing through an increase in LFA-1/ICAM-1 interaction. (A) OCI-AML-3 or NB4 cells were treated with 2 mM metformin for 3 days and later incubated with 20 µg/ml of an antibody (MAB139) blocking NKG2D activity before overnight incubation at 1:1 E:T ratio with e-NK cells. (B) The different cell lines were treated as in (A) but they were incubated with 15 µg/ml of a LFA-1 blocking construct. (C) OCI-AML-3 cells were incubated as in (A) but in the presence of anti-ULBP1 (10 µg/ml), anti-MICA/B (10 µg/ml) or anti-β2 integrin (2, 5–10 µg/ml) blocking antibodies. (D) NB4 cells were incubated with different concentrations of ICAM-1 blocking peptides. The specific killing of tumor cells (7-AAD+) was quantified by FACs. The data represent means ± SD; *p < 0.05, **p < 0.01, ***p < 0.001 Student's t-test compared to control cells or as depicted in the graphic.

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