Figure 4
H84T BanLec addition after infection prevented VZV, HCMV, and HSV-1 spread in skin. Adult skin explants were inoculated with VZV (A), HCMV (B), or HSV-1 (C) and then placed on NetWells over medium that contained H84T BanLec (10 µM) added according to the schedule on the x-axis (blue arrows). Control antiviral compounds (cidofovir, CDV, 18 µM; ganciclovir, GCV, 100 µM; acyclovir, ACV, 10 µM) were added at DPI 0. Virus spread was measured by bioluminescence imaging starting on DPI 1. Virus yield on subsequent days was calculated by dividing the average Total Flux (photons/sec/cm2/steradian) by the average Total Flux on DPI 1. Viral growth kinetics (left panels, each symbol is the average for the group) were analyzed for statistical significance on the last day (right panels), where each symbol represents one piece of skin and the bar is the mean of the group. The Vehicle and ACV groups in (C) are the same as in Fig. 3C, as these experiments were conducted together. Asterisks indicate significance between the treated groups and vehicle (*p < 0.05, **p < 0.01, ***p < 0.001). p < 0.01, one-way ANOVA, Dunnett’s post hoc test. N = 4–6 biological replicates.
