Figure 4 | Scientific Reports

Figure 4

From: Endothelial Notch signaling directly regulates the small GTPase RND1 to facilitate Notch suppression of endothelial migration

Figure 4

RND1 knockdown does not rescue Notch-mediated suppression of endothelial proliferation. (A) Transfection with a DLL4-myc expression construct induces DLL4 protein expression in HUVECs 3 days after lentivirus induction. All images are from the same blot, with uncropped version provided in Fig. S4. (B) RND1 siRNA robustly knocks down RND1 in HUVEC with normal levels of Notch signaling (RFP) or Notch signaling activated by DLL4-myc overexpression (DLL4). HUVECs were lentivirally transduced with RFP or DLL4 and transfected the next day with scrambled (siCNT) or siRNA targeting RND1 (siRND1). 3 days after lentivirus infection, HUVECs were harvested and analyzed by qPCR for RND1. (C) Treatment with siRNA1 does not grossly affect DLL4 levels in HUVEC treated as in (B). (D) Treatment with siRND1 does not restore proliferation in HUVEC where Notch signaling has been activated by DLL4. 104 HUVEC treated as in (B) were seeded onto 6-well plates cell and counted daily. Proliferation rate was normalized to counts at day 1.

Back to article page