Table 4 Proportions of reads supporting insertions.

From: Advanced sequencing approaches detected insertions of viral and human origin in the viral genome of chronic hepatitis E virus patients

Sequencing method

Insertion

Sample

Reads containing insertion/total reads

Percentage of reads containing insertion

Whole-genome NGS

AHNAKb

Sample 1

36,170/42,760

 ~ 85%

Sample 2

4,770/23,410

 ~ 20%

HEV–RdRpa

Sample 1

1,360/42,760

 ~ 3%

Sample 2

5,590 /23,410

 ~ 24%

Amplicon-based NGS

AHNAKb

Sample 1

620/15,980

 ~ 4%

Sample 1b

31,840/135,350

 ~ 23%

RPL18c, variant 1

Sample 1

5,210/15,980

 ~ 33%

Sample 1b

47,220/135,350

 ~ 35%

RPL18-2c, variant 2

Sample 1

0

0%

Sample 1b

1,580/135,350

 ~ 1%

HEV–RdRpa

Sample 1

20/15,980

 < 1%

Sample 1b

1,280/135,350

 ~ 1%

HEV–HVRa

Sample 1

0

0%

Sample 1b

814/135,350

 < 1%

  1. aUsing wbGER27 as reference (accession No: FJ705359.1); bhomo sapiens AHNAK nucleoprotein (accession No.: NG_051822.1); chomo sapiens ribosomal protein L18 (accession No.: L11566.1).