Figure 3

SARS-CoV-2 specific T cell and B cell responses in individuals who received the Gam-COVID-Vac. Ex vivo IFNγ ELISpot assays were carried out at 4 weeks (individuals who were vaccinated from Gam-COVID-Vac) (n = 40) in baseline uninfected individuals for overlapping peptides of spike protein, which were in two pools S1 and S2 (A). Intracellular cytokine staining was used to determine CD107a expression and IFNγ production at 4 weeks (n = 32) in CD4+ and CD8+ by flow cytometry (B). The number of antibody secreting B cells (ASCs) were determined at 4 weeks (n = 25) by doing B cell ELISpot assays (C). Wilcoxon matched pairs signed ranked test was used to find out the differences in ex vivo ELISpot responses for S1 and S2, and differences in CD107a expression and IFNγ production by CD4+ and CD8+ T cells and for B cell ELISpot assays. All tests were two sided. The error bars indicate the median and the interquartile ranges.