Figure 2

Representative example of FC histograms of capacitating and non-capacitating spermatozoa (donor 3), stained by four different fluorescent dyes. The Y-axis represents the number of spermatozoa labelled by the corresponding fluorescence depicted in the X-axis. (a) MQAE: black non-capacitated, yellow capacitated; (b) Fluo4: grey non-capacitated, blue capacitated; (c) sodium green staining: grey non-capacitated, orange capacitated; (d) progesterone-FITC staining: grey non-capacitated, blue capacitated.