Figure 1
X. laevis Il11ra.L functions as an Il11 receptor component. (A) Gene expression involved in Il11 signaling in the XTC-YF cell line. (B–D) Oocyte culture supernatants were subjected to SDS-PAGE, followed by (B) CBB staining and (C) Western blotting using anti-FLAG antibody and (D) mouse IgG2b isotype control. Size of the synthesized proteins estimated by their amino acid sequences was: Il11, 22.2 kDa; Lif, 25.5 kDa; and GST, 28.4 kDa. Arrowheads in (B) indicate the synthesized proteins. The 66.4-kDa bands (asterisk) in (B) the CBB staining represent BSA contained in the culture medium. (E–Q) Detection of XTC-YF P-Stat3 nuclear translocation by immunostaining. (Top) P-Stat3 signal is shown in red. (Middle) Nuclei were stained with DAPI. (Bottom) Merged images of P-Stat3 and DAPI staining. Data shown are representative of 3 independent experiments (except the experimental condition of Il11 50 ng/mL; n = 2). Scale bars: 50 µm. Raw images of the gel and blots are shown in Fig. S8.
