Figure 2

il11ra.L is required for X. laevis tadpole tail regeneration. (A) Typical examples of regenerated tails at 7 dpa of tadpoles injected with either (left) cas9 mRNA only, (center) cas9 mRNA and guide RNA #1, or (right) cas9 mRNA, and guide RNA #1 and #2. Dashed lines indicate the amputation site. Scale bars: 500 µm. (B) Regeneration outcomes of il11ra.L KD tadpoles. Tadpoles at 7 dpa were classified into 4 groups depending on their tail regeneration: Excellent, completely regenerated with all tissues; Good, with all tissues but short or bent tail; Partial, with tissue(s) defect; None, no regeneration. Numbers shown in the bar chart are numbers of tadpoles classified into each group. Statistical significance was assessed by Fisher’s exact test with the Benjamini–Hochberg method, *P < 0.05, **P < 0.01, ***P < 0.001. (C–J) Measurement of regenerated tails. (C) Regeneration outcomes of the KD experiment used for measurement. The same classification criteria as in (B) were used. cas9 group, n = 25; #1&#2 KD group, n = 32. ***P < 0.001 by Fisher’s exact test. (D) Schematic figure of the measurement. (E) Length of the regenerated tails. Bars indicate means. *P < 1 × 10^–10 by Welch's t-test. (F) Axis angle of the regenerated tails. The dispersion in the 2 groups was compared by F-test, †P < 1 × 10^–10. (G) Combined plot of length and axis angle of the regenerated tails. (H–J) Measured area of (H) whole regenerated tail, (I) muscle and (J) notochord in the regenerated tail. Bars indicate means. *P < 1 × 10^–9 by Welch's t-test.