Table 3 Enzyme leached out (%) at various incubation temperatures/times from immobilized SA-based (A8, A9), and CH-based (C6, C8) hydrogel samples.

From: Synthesis of two novel bio-based hydrogels using sodium alginate and chitosan and their proficiency in physical immobilization of enzymes

Incubation condition

Temperature (°C), 1 h

Time (min), 50 °C

 

40 °C

50 °C

60 °C

70 °C

30 min

60 min

90 min

120 min

180 min

Enzyme leached (%)

A8

7.8

8.2

9.6

12.7

7.4

8.2

9.6

11.8

16.3

A9

5.6

6.6

8.8

11.4

4.2

6.6

8.2

9.06

13.2

C6

13.0

17.3

22.1

26.2

15.93

17.3

17.96

28.7

36.6

C8

11.4

15.8

17.2

20.5

13.8

15.8

20.06

23.5

29.9

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