Figure 2

Correlation of spike-pseudotyped lentiviral neutralization, disease severity and serology results. (a) COVID-19 patients were classified according to their disease severity (0 = healthy controls, 1 = asymptomatic COVID-19 patients, 2 = hospitalized non-ICU COVID-19 patients, 3 = ICU COVID-19 patients, 4 = deceased COVID-19 patients). Correlation between spike-pseudotyped lentiviral neutralization and severity was assessed with simple linear regression (solid line) and 95% confidence bands (dashed line) of the best-fit line. The Spearman’s correlation coefficient (r) is shown. (b) The neutralization capacity in serum or plasma of healthy controls and COVID-19 patients was determined by spike-pseudotyped lentiviral neutralization assay and was expressed in terms of a percentage of neutralization. The six patient cohorts are presented in Table 1. The percentage of neutralizing samples is indicated. Samples were considered neutralizing when neutralization capacity was greater than 50% (dashed line). (a,b) Each dot represents one sample. *p < 0.05, ***p < 0.001, ****p < 0.0001 (Kruskal Wallis test and Dunn’s multiple comparisons test). (c) Correlations between spike-pseudotyped lentiviral neutralization and titers of IgG, IgM and IgA directed against RBD or N were assessed for asymptomatic COVID-19 patients, hospitalized COVID-19 patients (including non-ICU, ICU and deceased COVID-19 patients) and recovered patients. Spearman’s correlation coefficients (r) are shown and visualized by colour intensity. All correlations are significant (p < 0.05) except those indicated by "ns".