Figure 2 | Scientific Reports

Figure 2

From: Characterization and functional analysis of the adipose tissue-derived stromal vascular fraction of pediatric patients with osteogenesis imperfecta

Figure 2

Osteogenic gene expression profile of SVF cells obtained from healthy controls (HC) or OI patients at days 7 and 21 in culture. (A) Fold-change of gene expression of treated versus non-treated cells at day 7 of culture. (B) Fold-change of gene expression of treated versus non-treated cells at day 21 of culture. (C) Quantification of alizarin red staining via absorbance measurements displayed as fold-change of treated versus non-treated samples. (D) Representative images of alizarin red staining of treated and non-treated HC and OI samples. HC, n = 2; OI, n = 9; OI-COL1A1, n = 1; OI-COL1A2, n = 6; OI-WNT1, n = 1. ALP alkaline phosphatase; OCN osteocalcin. Unpaired t-test of genetic fold-change of HC versus OI patients showed similar expression for all genes tested.

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