Table 3 Multi-lineage cell culture media.
Media | Composition |
|---|---|
Osteogenic induction media | DMEM: F12 |
10% FBS | |
1% penicillin–streptomycin | |
1% antibiotic–antimycotic | |
10 nM dexamethasone | |
10 mM β-glycerol phosphate | |
50 µg/ml ascorbic-acid-2-phospahate | |
Osteogenic maintenance media | DMEM: F12 |
10% FBS | |
1% penicillin–streptomycin | |
1% antibiotic–antimycotic | |
10 nM dexamethasone | |
5 mM β-glycerol phosphate | |
50 µg/ml ascorbic-acid-2-phospahate | |
Adipogenic induction media | DMEM: F12 |
10% FBS | |
1% penicillin–streptomycin | |
1% antibiotic–antimycotic | |
10 µg/ml insulin | |
1 µM dexamethasone | |
500 µM 3-isobutyl-1-methylxanthine | |
Adipogenic maintenance media | DMEM: F12 |
10% FBS | |
1% penicillin–streptomycin | |
1% antibiotic–antimycotic | |
10 µg/ml insulin | |
Chondrogenic induction media | DMEM: F12 |
10% FBS | |
1% penicillin–streptomycin | |
1% antibiotic–antimycotic | |
10 nM dexamethasone | |
50 µg/ml ascorbic-acid-2-phospahate | |
10 ng/ml recombinant human TGF-β3 | |
40 μg/ml l-proline | |
50 μg/ml ITS liquid media supplement |
