Figure 7
Dose-dependent inhibition of SARS-CoV-2 PLpro proteolytic and DUB activity by aloin A and B. A serial dilution of aloin A and B ranging from 0–100 µM in assay buffer was used. The percent activity was calculated as described in Fig. 2 legend. DMSO (0.1%) with enzyme and 50µM of substrate served as positive control. Wells with 50µM of GRL-0617 compounds served as specificity controls for PLpro. Representative of three individual experiments with triplicate values were presented graphically (n = 3). Non-linear regression (curve fit) with four variable dose vs inhibition was used to calculate the IC50 values using GraphPad Prism.
