Figure 8 | Scientific Reports

Figure 8

From: Aloin isoforms (A and B) selectively inhibits proteolytic and deubiquitinating activity of papain like protease (PLpro) of SARS-CoV-2 in vitro

Figure 8

Time-dependent inhibition of SARS-CoV-2 PLpro proteolytic and DUB activity by aloin A and B. Aloin A and aloin B were tested for their time-dependent (0–18h) inhibitory activity at 50µM concentration against SARS-CoV-2 PLpro enzyme (A: proteolytic, B: DUB activity). The fluorescence intensity was used to calculate the percent enzymatic activity considering DMSO treated control as 100% activity. Blank values were subtracted before calculating the percent activity. DMSO (0.1%) with enzyme and 50µM of substrate served as positive control. Wells with 50µM of GRL0617 compounds served as specificity controls for PLpro. Representative of four experiments (n = 4) with triplicate values were presented graphically.

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