Figure 1
Experimental overview and physiological/immunological changes in the different groups. (A) Details of the sensitization and sublingual immunotherapy treatment for the different groups. *Challenge performed 1 week (1w) after the end of SLIT; #Were the challenge to be performed 5 weeks after the end of SLIT, as shown previously. (B) Overview of the experimental design in which mice were sensitized with Pru p 3 (Antigen-only) or Pru p 3 + LPS (additional three groups), then underwent sublingual immunotherapy, before being challenged one week after finishing treatment. (C) In vivo evaluation of anaphylaxis after SLIT with D1ManPrup3. Dots show decreases in body temperature 30–40 min after challenge with Pru p 3, 1w after ending the SLIT.. The individuals used for the subsequent RNA analysis are marked in black. (D) In vitro evaluation after SLIT with D1ManPrup3. Bars represent the mean serum level of Pru p 3-sIgE and -sIgG1 by ELISA in different groups (upper part) and the number of Pru p 3-specific IgE and IgG1 secreting cells by ELISpot assay for each group (lower part). Dots represent individual cases. Significant differences when P < 0.05 compared to Anaphylaxis group. The individuals used for the subsequent RNA analysis are marked in black.
