Figure 3
From: Hemangiosarcoma cells induce M2 polarization and PD-L1 expression in macrophages
HSA tumor cells and tumor infiltrating macrophages express PD-L1. (A) HE staining and immunohistochemistry of PD-L1 for ISOS-1 and canine HSA tumors. HE staining images are the same as in Fig. 2A because the same samples were used for this experiment. (B) Immunofluorescence assay for PD-L1 and Iba1 in ISOS-1 and canine HSA tumors. Arrows indicate the cells expressing both PD-L1 and Iba1. (C) The percentages of T cells infiltrating in canine HSA tumor tissues with or without PD-L1 expressions in macrophages or tumor cells. (D) Representative T cell staining images of canine HSA tumor tissues with or without PD-L1 expressions in macrophages. Scale = 125 μm. P-values were calculated by Mann–Whitney U test.
