Figure 4
From: Hemangiosarcoma cells induce M2 polarization and PD-L1 expression in macrophages
ISOS-1 cells induce PD-L1 expression in RAW264.7 cells. (A) Representative images of flow cytometry analysis for PD-L1 in ISOS-1 and JuB2 cell lines with/without IFNγ treatment. APC indicates PD-L1 expressions. (B) Quantitative analysis of A. (C) Gene expression levels of PD-L1 in untreated, LPS and IFN-treated, TGFβ-treated, and ISOS-1 conditioned media-treated RAW264.7 cells. (D) Representative images of flow cytometry analysis for PD-L1 in RAW264.7 cells cultured in normal or ISOS-1 conditioned media. (D) Quantitative analysis of (C). (E) Representative images of flow cytometry analysis for PD-L1 in mouse peritoneal macrophages cultured in normal or ISOS-1 conditioned media. (F) Quantitative analysis of (E). Data are presented as mean values ± s.d. ***P < 0.001, Tukey’s test.
