Figure 2 | Scientific Reports

Figure 2

From: Activation of the NLRP3 inflammasome and elevation of interleukin-1β secretion in infection by sever fever with thrombocytopenia syndrome virus

Figure 2

Induced upregulation or processing of pro-IL-1β, IL-18 and pro-caspase 1 in PBMCs infected with SFTSV. PBMCs were isolated from healthy individuals and infected with SFTSV at an MOI = 1 for 24, 48, or 72 h (A,C,E) or for 48 h at an MOI = 0.1, 1, or 2 (B,D,F). IL-1β and GAPDH mRNAs were quantified by RT-PCR with specific primers for IL-1β and GAPDH, respectively (A,B). IL-1β and IL-18 levels in the culture medium of PBMCs infected with SFTSV were determined by ELISA (C,D,E,F). Upregulation of pro-IL-1β and processing of cleaved caspase-1 (p20) were determined by western blot with specific antibodies for pro-IL-1β and cleaved caspase 1 (p20), respectively (G,H).

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