Figure 3
From: Relationship between oxidative stress and lifespan in Daphnia pulex
Oxidative damage to cellular proteins. (A) Protein carbonyl levels were measured with the Oxyblot kit (Millipore). Using total cellular extracts, the carbonyl groups in the protein side chains were derivatized to 2,4-dinitrophenyl hydrazine (DNP). Western blot analysis was performed with an antibody against DNP. Equal loading was assessed using an anti-β actin antibody (Sigma). Y: young (1 wk for both clones), M: middle aged (2 wk for short-lived RW20 and 4 wk for long-lived XVI-11) and O: old age (3 wk for short-lived RW20 and 8 wk for long-lived XVI-11). (B) Bar graph represents the signals obtained from the analysis of the average of 3 blots from various samples after normalization to the β-actin bands. Blue corresponds to short-lived RW20 and orange corresponds to the long-lived XVI-11.
