Figure 4

Oxidative damage to MT proteins. (A) Protein carbonyl levels were measured with the Oxyblot kit (Millipore). Using mitochondrial protein extracts, the carbonyl groups in the protein side chains were derivatized to 2,4-dinitrophenyl hydrazine (DNP). Western blot analysis was performed with an antibody against DNP. Equal loading was assessed using an anti-β actin antibody (Sigma). Lane 1: MT extract from mixed age populations of WF6 with about equal individuals from ages 1 wk-3 wk of the short-lived WF6 and lane 2: MT extract from mixed age populations of XVI-11 with about equal individuals from ages 1 wk-3 wk of the long-lived XVI-11. (B) Bar graph represents the signals obtained from the analysis of the average of 3 blots after normalization to the β-actin bands. Blue corresponds to short-lived WF6 and orange corresponds to the long-lived XVI-11.