Figure 6

Comparison of hydrogen peroxide levels in short-lived RW20 and long-lived XVI-11. Hydrogen peroxide levels were determined at the indicated ages for short-lived RW20 and using a kit available through Pierce. Before the kit, which uses a colorimetric assay, was used with samples from aged Daphnia, the kit was standardized for use with Daphnia samples (Data not shown). All reactions were performed in a 96 well plate that was read on a BioTek Plate reader at a wavelength of 560 nm using the Gen5 software that was included as part of the Bio-Tek plate reader. Blue corresponds to short-lived RW20 and orange corresponds to the long-lived XVI-11. Error bars indicate the standard error of the mean from 3 experiments. Student T-tests were performed and the p values are as indicated, ns means not significant.