Figure 6 | Scientific Reports

Figure 6

From: Dual-sgRNA CRISPR/Cas9 knockout of PD-L1 in human U87 glioblastoma tumor cells inhibits proliferation, invasion, and tumor-associated macrophage polarization

Figure 6

PD-L1 knockout inhibits U87 cell invasion and migration. (A) A scratch assay was performed to examine U87 cell invasion after PD-L1 knockout by Cas9-g82/165 + HDR. Invasion of U87 cells into the scratched area was analyzed within the black lines. U87 cells treated with Cas9-g82/165 + HDR were less invasive compared to the control. (B) A migration assay using a 3 µm insert was used to analyze migratory potential. Cells were plated in the insert, and their migration to the plate surface was monitored over three days. The number of cells that migrated were counted, and results were normalized across 3 experiments. The data suggests Cas9-g82/165 + HDR slowed migration of U87 cells compared to control. The data represent the mean ± SEM and statistical significance was evaluated with a t-test (B). *p < 0.05. Scale bar = 200 µm.

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