Figure 2
Summarized data of the effect of PAF (10−9–10−6 M) and its vehicle (0.25% bovine serum albumin) on the basal tone (a) and amplitude (b)/frequency (c) of spontaneous contractions in the isolated guinea pig urinary bladder smooth muscle shown in Fig. 1. Basal tone increases show basal tone changes before and after administration of PAF or its vehicle (a). Spontaneous contraction activities analyzed over 3 min during the following periods were calculated: immediately before administration of PAF or its vehicle (Ctrl, control); 7–10 min (10), 17–20 min (20), 27–30 min (30), 37–40 min (40), 47–50 min (50), and 57–60 min (60) after administration of PAF or its vehicle (b,c). Data are expressed as the means ± SEM (each n = 5). *P < 0.05; **P < 0.01 vs. the corresponding vehicle value; #P < 0.05; ##P < 0.01 vs. the corresponding control value (two-way ANOVA followed by Dunnett’s test). PAF platelet-activating factor, ACh acetylcholine.
