Figure 6

Myelination of sciatic nerves of Pbrm1 cKO mice at P21. (A) Overview of sciatic nerve tissue from control (Ctrl) and Pbrm1 cKO mice at P21 after PPD staining. (B) Quantification of the percentage of myelinated axons in sciatic nerves of control and Pbrm1 cKO mice at P21 (shown as mean ± SEM, n = 3 per genotype). (C–E) G-ratio determination for myelinated axons in sciatic nerves of control and Pbrm1 cKO mice after binning by axon diameter (C), as scatter blot for single fibers (D, based on n = 150 fibers per genotype) or as average (E, shown as mean ± SEM, n = 3 per genotype). (F) Percental size distribution of axons (larger than 1 µm) in sciatic nerves of control and Pbrm1 cKO mice at P21 after binning by diameter (n = 150 fibers per genotype). (G) High resolution electron microscopic pictures of sciatic nerve tissue from control and Pbrm1 cKO mice at P21 depicting a representative Remak bundle (upper panels) and myelinated large calibre axons (lower panels). (H) Determination of Iba1-positive macrophages per sciatic nerve cross-section in control and Pbrm1 cKO mice from P0 until P60 (mean ± SEM, n = 3 per genotype). (I) Immunohistochemical stainings of nerve sections of control and Pbrm1 cKO mice from P0 until P60 with antibodies directed against Iba1. Scale bar: 10 µm (A, I), 3 µm (G). Statistical significance between genotypes was determined separately for each time point, bin and marker by unpaired two-tailed Student’s t-test. However, no significant difference was detected.