Figure 1

Susceptibility of antimicrobials to human serum inhibition. (a) Effect of human serum on the bactericidal activities of antimicrobials against E. coli (106 cfu/ml). Values are the mean ± SD (n = 3). Mann–Whitney tests were performed to determine the significance of the difference. * Indicates P ≤ 0.05 compared with PBS group. (b) The band shift of antimicrobials (4 µg each) in 10 µl diluted human serum analyzed by 8% horizontal native PAGE and Coomassie blue staining. Arrow indicates control (non-shift) AMP. PBS, phosphate-buffered saline; 5% HS, 5% human serum in PBS (v/v).