Figure 6 | Scientific Reports

Figure 6

From: The lipid components of high-density lipoproteins (HDL) are essential for the binding and transportation of antimicrobial peptides in human serum

Figure 6

Translocation of AMP from HDL-AMP complex to bacteria. (a) Dynamic binding between HDL and AMP. HDL was pulled down by immobilized AMPs, then incubated with twofold amount of free AMP for 30 min at 25 °C. The residual proteins remained on the beads were analyzed by 15% non-reducing SDS-PAGE. (b) Translocation of AMP to bacteria. The AMP (2.5 µg) alone or in complex with HDL (5 µg) was incubated with E. coli (108 cfu) for 5 min at 25 °C before centrifugation. The proteins in the supernatant (S) and pellet (P) were analyzed by 15% non-reducing SDS-PAGE and Coomassie blue staining. The AMPs translocated to bacteria or remained in soluble complex were shown in solid and dotted lines, respectively.

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