Figure 13

Effect of SIN-1 on scratch wound regression by ECFCs in vitro. Bright field phase contrast microscopic images (10x) of in vitro scratch wound assay at 03 different time points (0 h, 04 h and 08 h) is depicted in (A–F). Images (A–C) shows 10 × image of wound closure in untreated; (D-F) shows wound closure in SIN-1 treated group at three different time points 0 h, 04 h and 08 h; Scale bar 100 µm; (H) shows % in vitro scratch wound closure by ECFCs at two different time points from 0 to 04 h and 04 to 08 h in untreated and SIN-1 treated group (n = 3 for each group). Student t-test was used to assess the significance difference. Data is represented as mean ± SE. Error bar represent standard error of mean; dots represent average values of the parameters obtained from experimental duplicates; p ≤ 0.05 considered statistically significant.