Figure 1
Testosterone is significantly lower in females whereas estradiol levels are similar between sex groups. (A) Bar graph shows higher testosterone levels in all sexes, except for female rat pups (F: 1.01 ± 0.09 ng/mL, n = 15 rats from 3 litters; M: 3.44 ± 0.44 ng/mL, n = 15 rats from 3 litters; A: 4.96 ± 0.95 ng/ml, n = 14 rats from 4 litters; TFM: 4.64 ± 0.65 ng/Ml, n = 15 rats from 5 litters; One-way ANOVA with Tukey’s post hoc analysis, F × M: p = 0.028; F × A: p < 0.001; F × TFM: p < 0.001). (B) Bar graph shows no significant differences in levels of estradiol between all groups (F: 34.0 ± 1.81 pg/mL, n = 15 rats from 3 litters; M: 33.4 ± 2.14 pg/mL, n = 15 rats from 3 litters; A: 47.1 ± 5.47 pg/mL, n = 14 rats from 4 litters; TFM: 42.1 ± 1.18 pg/mL, n = 15 rats from 5 litters; One-way ANOVA with Tukey’s post hoc analysis, F × M: p = 0.995; F × A: p = 0.080; F × TFM: p = 0.140). Dots represent individual data points. M males, F females, A andro/testosterone-treated females, TFM testosterone-insensitive males. Graphs represent mean ± SEM.
