Figure 5
Perinatal testosterone negatively correlates with BDNF expression levels during the first postnatal week. (A) Western blot analysis of mature BDNF (14 kDa) expression levels in the hippocampus of different experimental groups at P7. Each lane represents a different animal. Blot shows representative samples of different sex groups (images were cropped from different gels). (B) Quantification revealed that the expression of mature BDNF is significantly lower in male when compared to female and testosterone-insensitive male rat pups (F: 1.21 ± 0.14 au, n = 13 rats from 4 litters; M: 0.68 ± 0.09 au, n = 10 rats from 4 litters; A: 0.74 ± 0.12 au, n = 6 rats from 3 litters; TFM: 1.4 ± 0.19 au, n = 6 rats from 3 litters; One-way ANOVA with Tukey’s post hoc analysis, F × M: p = 0.009; TFM × M: p = 0.007). Dots represent individual data points. F females, M males, A andro/testosterone-treated females, T TFM/testosterone-insensitive males. Graphs represent mean ± SEM.
