Figure 6
Testosterone signaling is associated with lower sIPSC frequency in young adult CA1 pyramidal neurons. (A) Representative traces of mIPSCs from CA1 pyramidal neurons. (B) mIPSC frequency (F: 2.01 ± 0.25 Hz, n = 9 cells, 4 rats from 3 litters; M: 1.97 ± 0.28 Hz, n = 12 cells, 5 rats from 3 litters; A: 1.73 ± 0.20 Hz, n = 9 cells, 4 rats from 3 litters; TFM: 1.95 ± 0.28 Hz, n = 9 cells , 4 rats from 3 litters; One-way ANOVA with Tukey’s post hoc analysis, p = 0.985) and (C) amplitude (F: 33.10 ± 2.10 pA, n = 9 cells, 4 rats from 3 litters; M: 31.70 ± 2.27 pA, n = 12 cells, 5 rats from 3 litters; A: 30.28 ± 2.52 pA, n = 9 cells, 4 rats from 3 litters; TFM: 31.92 ± 3.20 pA, n = 9 cells,4 rats from 3 litters; One-way ANOVA with Tukey’s post hoc analysis, p = 0.849) did not differ in hippocampal CA1 pyramidal neurons between the sex groups. (D) Representative traces of sIPSCs from CA1 pyramidal neurons. (E) Sex differences were found in the frequency of sIPSC (F: 10.40 ± 1.03 Hz, n = 18 cells, 7 rats from 4 litters; M: 5.23 ± 0.52 Hz, n = 17 cells, 6 rats from 3 litters; A: 5.42 ± 0.58 Hz, n = 16 cells, 6 rats from 3 litters; TFM: 11.90 ± 2.64 Hz, n = 14 cells, 5 rats from 3 litters; One-way ANOVA with Tukey’s post hoc analysis, F × M: p = 0.002; TFM × M: p = 0.021; F × A: p = 0.004; TFM × A: p = 0.030). (F) Conversely, no sex differences were found in the amplitude of sIPSC (F: 83.98 ± 9.48 pA, n = 18 cells, 7 rats from 4 litters; M: 59.13 ± 7.00 pA, n = 17 cells, 6 rats from 3 litters; A: 62.50 ± 5.45 pA, n = 16 cells, 6 rats from 3 litters; TFM: 68.74 ± 4.43, n = 14 cells, 5 rats from 3 litters; One-way ANOVA with Tukey’s post hoc analysis, p = 0.067) in the four experimental groups. Dots represent individual data points. F females, M males, A andro/testosterone-treated females, TFM testosterone-insensitive males. Graphs represent mean ± SEM.
