Figure 3
Experimental validation of cell-specific expression buffering between FAM50A and FAM50B, and POP7 and RPP25. (A) C-score plot of the highest C-score gene pair, FAM50A (dependency score—D.S.) and FAM50B (G2) expression, labeled with the U2OS (predicted not synergistic) and MCF7 (predicted synergistic) cell lines. (B) Relative cell growth between 0 and 90 h based on fold-change in confluency of the U2OS and MCF7 cell lines with or without FAM50A or FAM50B suppression. Bliss scores indicate strength of synergy between double suppression of FAM50A and FAM50B compared to either gene alone. Error bars indicate standard deviation of six technical repeats. (C) C-score plot of the non-duplicated gene pair, i.e., POP7 dependency score and RPP25 gene expression, labeled with the LN18 (predicted not synergistic) and HT29 (predicted synergistic) cell lines. (D) Relative cell growth between 0 and 90 h based on fold-change in confluency of the HT29, U2OS and LN18 cell lines with or without shRNA-based POP7 or RPP25 suppression. Bliss scores indicate strength of synergy between double suppression of POP7 and RPP25 compared to either gene alone. Error bars indicate standard deviation of six technical repeats.
