Figure 1 | Scientific Reports

Figure 1

From: Humanization of a strategic CD3 epitope enables evaluation of clinical T-cell engagers in a fully immunocompetent in vivo model

Figure 1

Structural rationalization for the hCD3E-epi mouse model. (a) Alignment of mouse and human CD3E to inform the design of the mouse model. (SP signal peptide, EC extracellular domain, TM transmembrane domain, IC intracellular domain.) Residues that are involved in binding to human CD3δ or human CD3γ are highlighted (blue). (b) HDX data for the anti-human CD3 molecules (red) mapped onto the sequence of human CD3E. (c) Structural model of human  CD3E alone in the membrane. Colors are N-terminal epitope that we replaced (orange), other human-cyno identical residues (red), human insertion in CD3 (white). (d) Human CD3E/D in complex with the TCR in the membrane. Only one CD3E (epsilon in complex with delta) in the TCR complex is shown, but the other (CD3 epsilon-gamma complex) looks similar. Models were generated using PBD IDs 6JXR and 2MLR.

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